Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells and Corynebacterium glutamicum strain ATCC 13032 | Rhodopseudomonas palustris |
Protein Variants | Comment | Organism |
---|---|---|
H15K | the mutant exhibits a 6.0°C higher melting temperature and shows a 10.3fold increase in specific activity after 1 h incubation at 37°C compared to the wild type enzyme | Rhodopseudomonas palustris |
H15R | the mutant shows an increase in specific activity after 1 h incubation at 37°C compared to the wild type enzyme | Rhodopseudomonas palustris |
H176D | the mutant shows an increase in specific activity after 1 h incubation at 37°C compared to the wild type enzyme | Rhodopseudomonas palustris |
H206F | the mutant shows an increase in specific activity after 1 h incubation at 37°C compared to the wild type enzyme | Rhodopseudomonas palustris |
H206Y | the mutant shows an increase in specific activity after 1 h incubation at 37°C compared to the wild type enzyme | Rhodopseudomonas palustris |
H216D | inactive | Rhodopseudomonas palustris |
H236D | the mutant shows an increase in specific activity after 1 h incubation at 37°C compared to the wild type enzyme | Rhodopseudomonas palustris |
H293Y | the mutant shows a decrease in specific activity after 1 h incubation at 37°C compared to the wild type enzyme | Rhodopseudomonas palustris |
H29R | the mutant exhibits a 2.3°C higher melting temperature and shows a 6.7fold increase in specific activity after 1 h incubation at 37°C compared to the wild type enzyme | Rhodopseudomonas palustris |
H304M | the mutant shows a strong increase in specific activity after 1 h incubation at 37°C compared to the wild type enzyme | Rhodopseudomonas palustris |
H304Q | the mutant shows a decrease in specific activity after 1 h incubation at 37°C compared to the wild type enzyme | Rhodopseudomonas palustris |
H304V | the mutant shows a decrease in specific activity after 1 h incubation at 37°C compared to the wild type enzyme | Rhodopseudomonas palustris |
H322L | the mutant shows a severe decrease in specific activity after 1 h incubation at 37°C compared to the wild type enzyme | Rhodopseudomonas palustris |
H322V | the mutant shows a severe decrease in specific activity after 1 h incubation at 37°C compared to the wild type enzyme | Rhodopseudomonas palustris |
H385R | the mutant shows a decrease in specific activity after 1 h incubation at 37°C compared to the wild type enzyme | Rhodopseudomonas palustris |
H39Y | the mutant shows an increase in specific activity after 1 h incubation at 37°C compared to the wild type enzyme | Rhodopseudomonas palustris |
H61D | the mutant shows a severe decrease in specific activity after 1 h incubation at 37°C compared to the wild type enzyme | Rhodopseudomonas palustris |
H61N | the mutant shows a decrease in specific activity after 1 h incubation at 37°C compared to the wild type enzyme | Rhodopseudomonas palustris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hemin | 27% residual activity at 0.01 mM | Rhodopseudomonas palustris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01513 | - |
succinyl-CoA | mutant enzyme H15K, at pH 7.5 and 37°C | Rhodopseudomonas palustris | |
0.0214 | - |
succinyl-CoA | wild type enzyme, at pH 7.5 and 37°C | Rhodopseudomonas palustris | |
0.02227 | - |
succinyl-CoA | mutant enzyme H29R, at pH 7.5 and 37°C | Rhodopseudomonas palustris | |
2.31 | - |
glycine | wild type enzyme, at pH 7.5 and 37°C | Rhodopseudomonas palustris | |
2.63 | - |
glycine | mutant enzyme H29R, at pH 7.5 and 37°C | Rhodopseudomonas palustris | |
3.25 | - |
glycine | mutant enzyme H15K, at pH 7.5 and 37°C | Rhodopseudomonas palustris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinyl-CoA + glycine | Rhodopseudomonas palustris | - |
5-aminolevulinate + CoA + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodopseudomonas palustris | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His-Trap column chromatography | Rhodopseudomonas palustris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinyl-CoA + glycine | - |
Rhodopseudomonas palustris | 5-aminolevulinate + CoA + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ALA synthase | - |
Rhodopseudomonas palustris |
ALAS | - |
Rhodopseudomonas palustris |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
after 60 min incubation at 37°C, the residual activity of wild type dramatically drops to 8% of the original activity | Rhodopseudomonas palustris |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.74 | - |
succinyl-CoA | wild type enzyme, at pH 7.5 and 37°C | Rhodopseudomonas palustris | |
3.1 | - |
succinyl-CoA | mutant enzyme H15K, at pH 7.5 and 37°C | Rhodopseudomonas palustris | |
3.2 | - |
succinyl-CoA | mutant enzyme H29R, at pH 7.5 and 37°C | Rhodopseudomonas palustris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Rhodopseudomonas palustris |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.88 | - |
glycine | mutant enzyme H15K, at pH 7.5 and 37°C | Rhodopseudomonas palustris | |
1.18 | - |
glycine | wild type enzyme, at pH 7.5 and 37°C | Rhodopseudomonas palustris | |
1.22 | - |
glycine | mutant enzyme H29R, at pH 7.5 and 37°C | Rhodopseudomonas palustris | |
130 | - |
succinyl-CoA | wild type enzyme, at pH 7.5 and 37°C | Rhodopseudomonas palustris | |
140 | - |
succinyl-CoA | mutant enzyme H29R, at pH 7.5 and 37°C | Rhodopseudomonas palustris | |
230 | - |
succinyl-CoA | mutant enzyme H15K, at pH 7.5 and 37°C | Rhodopseudomonas palustris |